Inadvertent transgenesis by conventional ICSI in mice

Inadvertent transgenesis by conventional ICSI in mice.

BACKGROUND ICSI is a relatively new treatment for human male-related infertility, as well as an efficient method for the production of transgenic animals by injecting into the oocyte sperm previously incubated with foreign DNA. As semen samples collected in human infertility clinics are frequently contaminated with bacteria, one risk associated with the ICSI procedure is the injection of foreig...

Generation of YAC transgenic mice by ICSI

Rescue of tropomyosin-induced familial hypertrophic cardiomyopathy mice by transgenesis.

Familial hypertrophic cardiomyopathy (FHC) is a disease caused by mutations in contractile proteins of the sarcomere. Our laboratory developed a mouse model of FHC with a mutation in the thin filament protein alpha-tropomyosin (TM) at amino acid 180 (Glu180Gly). The hearts of these mice exhibit dramatic systolic and diastolic dysfunction, and their myofilaments demonstrate increased calcium sen...

Site-specific integrase-mediated transgenesis in mice via pronuclear injection.

Microinjection of recombinant DNA into zygotic pronuclei has been widely used for producing transgenic mice. However, with this method, the insertion site, integrity, and copy number of the transgene cannot be controlled. Here, we present an integrase-based approach to produce transgenic mice via pronuclear injection, whereby an intact single-copy transgene can be inserted into predetermined ch...

Study of zoonotic bacteria in conventional laboratory mice breeding colonies

To investigate the status of conventional laboratory mice colonies for zoonotic bacteria, three mice colonies, NIH, NMRI and Balb/c strains of Razi institute were studied for five zoonotic bacteria including Pasteurella pneumotropica, Salmonella typhimurium, Salmonella enteritidis, Streptobacillus moniliformis, Streptococcus Pneumonia. Study populations were 74 mice of premature, mature and pos...